Biochemistry 2
| Lecturer | Masayoshi MAESHIMA, Professor |
|---|---|
| Department | School of Agricultural Sciences / Graduate School of Bioagricultural Sciences, 2013 Spring |
| Recommended for: | School of Agricultural Sciences 2nd year students (2・1.5 hours / session One session / week 15 weeks / semester) |
Course Overview
All organisms take foods and use them to get elements for their own components, such as proteins, and energy through various metabolisms including glycolysis, citric acid cycle, and energy-yielding metabolism.
The lecture provides basic information on chemical characteristics of the enzyme reactions, the detailed reactions of the glycolysis and citric acid cycle, biochemical regulation, and their physiological significance. Also this course examines how cells transduce energy obtained from glucose to ATP production through oxidative phosphorylation.
Course Aims
Through the lecture, students will be able to:
- understand the chemical logic and biochemical reactions of sugar metabolism and oxidative phosphorylation
- define physicochemical logic of bioenergetics
- understand general properties of enzymes
- describe and calculate the parameters of enzyme reactions
Keywords
Metabolism, glycolysis, citric acid cycle, urea cycle, enzyme kinetics, oxidative phosphorylation, ATP synthesis
Textbook
Lehninger Princeples of Biochemistry, Sixth edition (Fifth edition for Japanese translation)
References
The instructors will pass out handouts in class. Other reading materials will be introduced by the instructors
Related Classes
Biochemistry 1, Basic chemistry II
Course Schedule
| Part1 Instructor: MAESHIMA | |
|---|---|
| 1 | General introduction |
| 2 | Cell compartments and their biochemical roles |
| 3 | Biochemical methods to analyze proteins (introduction for genomes, subcellular fractionation, protein purification, and functional analysis) |
| 4 | Enzyme kinetics as an approach to understanding metabolism (Enzyme kinetics, Michaelis-Menten equation, and Enzyme inhibitors) |
| Part2 Instructor: HORIO | |
| 5 | Glycolysis |
| 6 | Regulation of metabolic pathway |
| 7 | Pentose phosphate pathway of glucose oxidation |
| 8 | Citric acid cycle |
| 9 | Oxidation of fatty acid |
| 10 | Amino acid oxidation and urea cycle |
| Part3 Instructor: MAESHIMA | |
| 11 | Enzyme inhibitors and kinetic tests |
| 12 | Coenzymes and cofactors |
| 13 | Bioenergetics: ATP and high-energy phosphate compounds |
| 14 | Oxidative phosphorylation |
| 15 | Energy efficiency of ATP synthesis |
Grading
Attendance (20%), final exam (80%)
Last updated
March 17, 2020