Biochemistry I

Course Overview

Biochemistry is the study of the chemistry of life processes. It is the unifying principle underlying biological diversity.

Through biochemistry, we learn microscopic processes such as protein phosphorylation that regulates many signaling cascades in the cells. We also learn macroscopic processes such as evolutionarily conservation of a protein family which works similarly from bacteria to humans.

This course covers thermodynamics of a system, building blocks of principle biomolecules such as DNA and proteins, and the relationship between their structures and functions. Together, we aim to understand the mechanisms of action of the biomolecules, which allows an organism to live.

Textbooks:

Lehninger Principles of Biochemistry, the 7th edition Principles of Biochemistry Voet & Voet, the 4th edition Biochemistry Stryer, the 8th edition

Evaluation

Midterm and final exam each  40 x 2 = 80%
Quizzes  10%
Activities + Participation  10%

Class schedule

Month	Day	Topic
10	15	Introduction: chemical and cellular foundations of life
	22	Thermodynamics: physical foundation of life
	29	Water: Physical & chemical properties of water
11	5	Amino Acids
	12	Proteins: 2D structures
	19	Proteins: 3D structures
	26	Review session
	29	EXAM
12	3	Proteins in action: Hemoglobin
	10	Tools to study protein functions
	17	Protein function: enzyme I
	26	Protein function: enzyme II
1	8	Nucleic acids
	21	Nucleic acids: tools to understand
	28	Review session
2	4	EXAM

Lecture Notes

• 01_Introduction
• 02_Thermodynamics
• 03_Water
• [04_amino acids](https://ocw.nagoya-u.jp/files/689/4_amino acids.pdf)
• [05_Protein structures 2D](https://ocw.nagoya-u.jp/files/689/5_Protein structures 2D.pdf)
• [06_Protein structures 3D](https://ocw.nagoya-u.jp/files/689/6_Protein structures 3D.pdf)
• 07_HG
• 08-01_Chromatography
• [08-02_Affinity Chromatography](https://ocw.nagoya-u.jp/files/689/8-02_Affinity Chromatography.pdf)
• 08-03_BIOCHEM anh
• 08-04_ELISA
• 08-05_NMR presentation
• [08-06_Protein NMR](https://ocw.nagoya-u.jp/files/689/8-06_Protein NMR.pdf)
• [08-07_PROTEIN SEQUENCING BACH](https://ocw.nagoya-u.jp/files/689/8-07_PROTEIN SEQUENCING.pdf)
• [08-08_Protein sequencing](https://ocw.nagoya-u.jp/files/689/8-08_Protein sequencing(2).pdf)
• [08-09_western blot Cindy](https://ocw.nagoya-u.jp/files/689/8-09_western blot.pdf)
• [08-10_western blot may](https://ocw.nagoya-u.jp/files/689/8-10_western blot(2).pdf)
• [09_enzymes I](https://ocw.nagoya-u.jp/files/689/9_enzymes I.pdf)
• [10_enzyme II](https://ocw.nagoya-u.jp/files/689/10_enzyme II.pdf)
• [11_nucleic acids](https://ocw.nagoya-u.jp/files/689/11_nucleic acids.pdf)
• [12-01_Biochem 1 PCR](https://ocw.nagoya-u.jp/files/689/12-01_Biochem 1 PCR.pdf)
• [12-02_biochem ppt (dna sequencing)](https://ocw.nagoya-u.jp/files/689/12-02_biochem II (DNA sequencing).pdf)
• [12-03_Recombinant DNA and cloning](https://ocw.nagoya-u.jp/files/689/12-03_Recombinant DNA and cloning.pdf)
• [12-04_Biochem_recombination and cloning](https://ocw.nagoya-u.jp/files/689/12-04_Biochem_recombination and cloning.pdf)
• [12-05_DNA sequencing Bach](https://ocw.nagoya-u.jp/files/689/12-05_DNA sequencing.pdf)
• [12-06_Gene Editing](https://ocw.nagoya-u.jp/files/689/12-06_Gene Editing.pdf)
• 12-07_PCR
• [12-08_Gene Editing](https://ocw.nagoya-u.jp/files/689/12-08_Gene Editing.pdf)
• [12-09_RNA interfernce](https://ocw.nagoya-u.jp/files/689/12-09_RNA interfernce.pdf)
• 12-10_RNA-interference
• 13_review
• [Answers for HW](https://ocw.nagoya-u.jp/files/689/answers for HW.pdf)

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